Plasmid_Backbone

Part:BBa_K1641999

Designed by: Chunyang Ni   Group: iGEM15_SYSU_CHINA   (2015-09-11)


pAUR135 shuttle vector RFC10 optimized

pAUR135 RFC25 is a modified shuttle vector including DNA sequence to remove the vector region containing selectable marker AUR1-C from transformants of S.cerevisiae by this vector which allows repeatedly transformation. With optimization, K1641999 can be applied on RFC standard.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5858
    Illegal suffix found at 5880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5858
    Illegal NheI site found at 2517
    Illegal NheI site found at 2796
    Illegal SpeI site found at 5881
    Illegal PstI site found at 5895
    Illegal NotI site found at 5864
    Illegal NotI site found at 5888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5858
    Illegal BamHI site found at 2975
    Illegal BamHI site found at 5844
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5858
    Illegal suffix found at 5881
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5858
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5873
    Illegal SpeI site found at 5881
    Illegal PstI site found at 5895
    Illegal AgeI site found at 4808
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1197
    Illegal BsaI.rc site found at 2857
    Illegal BsaI.rc site found at 5411
    Illegal SapI site found at 4246
    Illegal SapI.rc site found at 2278


[edit]
Categories
Parameters
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